The interaction between an Ebola virus protein and a protein in human cells that may be an important key to unlocking the pathway of replication of the killer disease in human hosts has been identified.
A team of researchers at Texas Biomedical Research Institute were part of the nationwide collaborative with scientists at Gladstone Institutes, UC San Francisco and Georgia State University for the study recently published in the journal Cell.
Texas Biomed Staff Scientist Olena Shtanko, Ph.D., describes this new work as a “turning point for understanding how replication of Ebola virus is modulated.” Her role in the project was to validate and test whether the interaction between an Ebola virus protein called VP 30 and a host (human) protein called RBBP6 had involvement in the life cycle of the virus. Shtanko worked on this project while in the lab of Dr. Robert Davey, a former Texas Biomed Scientist, now at Boston University. Earlier research by scientists in California used a protein interaction map to narrow down host and virus protein interactions and then using a yeast system and an artificial proxy virus system proved the theory of this particular protein-protein interaction. However, scientists needed to use replicating virus and human immune cells to test the clinical significance of the finding. “The interaction is important if you can show functional significance of what it does to the virus in cells that have clinical relevance,” Shtanko stressed. “If you can figure out the mechanism within these cells, then you can potentially manipulate it and stop the disease progression.” Texas Biomed Staff Scientist Eusondia Arnett, Ph.D., and Texas Biomed President and CEO Dr. Larry Schlesinger – both tuberculosis researchers – have expertise in working with human macrophage (immune) cells drawn from donated blood samples. “We were able to capitalize on our experience with macrophages to over- and under-express the RBBP6 (host) protein to create an effective model for this important Ebola virus research,” Arnett said. By over- and under-expressing the RBBP6, Shtanko was able to test what impact the protein had on the growth of Ebola virus in the macrophages. Shtanko said the results were striking.
When the host protein was under-expressed, the viral replication went up exponentially. She found similar results when working with vascular cells, which are also key to Ebola virus replication in an infected patient.